Anti-FcεRIα antibodies – functional grade, human

Anti-FcεRIα antibodies – functional grade, human

Clone: CRA1
Clone CRA1 detects the alpha subunit of the human high-affinity Fc receptor for IgE. The receptor is composed of one alpha-, one beta-, and two disulfid-linked gamma subunits, of which the alpha subunit binds IgE. FcεRIα is expressed on mast and basophilic cells and is upregulated in the presence of IgE, and is found on a subset of blood dendritic cells. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions. Clone CRA1 does not inhibit binding of IgE, it induces mast cell activation, followed by histamin release.

Applications

This pure – functional grade antibody is suited for cell culture functional assays, for example, blocking of receptor-ligand binding.

Alternative names

FCER1A, Fce1a, FcERI

Technical specifications

  • Antigen: FcεRIα
  • Clone: CRA1
  • Isotype: mouse IgG2b
  • Alternative names of antigen: FCER1A, Fce1a, FcERI
  • Entrez Gene ID: 2205
  • Molecular mass of antigen [kDa]: 27
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates:
  • Selected references

    1. Hasegawa, S. et al. (1999) Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its`intracellular expression in human megakaryocytes. Blood 93: 2543-2551
    2. Suzukawa, M. et al. (2005) IgE- and FcepsilonRI-mediated migration of human basophils. Int. Immunol. 17: 1249-1255
    3. Hakimi, J. et al. (1990) The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding. J. Biol. Chem. 265: 22079-22081
    4. Hammad, H. et al. (2010) Inflammatory dendritic cells–not basophils–are necessary and sufficient for induction of Tʜ2 immunity to inhaled house dust mite allergen. J. Exp. Med. 207: 2097-2111
    5. Takai, T. et al. (2000) Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Biosci. Biotechnol. Biochem. 64: 1856-1867
    6. Yokoi, H. et al. (2008) Inhibition of Fcepsilon RI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement. J. Allergy Clin. Immunol. 121: 499-505
Product options: 1
130-096-004

Anti-FcεRIα pure - functional grade, human

100 µg in 1 mL
EUR 165,00