StraightFrom™ Buffy Coat CD14 MicroBead Kit, human

StraightFrom™ Buffy Coat CD14 MicroBead Kit, human

The StraightFrom™ Buffy Coat CD14 MicroBead Kit has been developed for the rapid positive selection of CD14
+
cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
®
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat.

Background information

The CD14 antigen belongs to the LPS receptor complex. It is strongly expressed on most monocytes and macrophages and weakly on neutrophils.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom™ Buffy Coat CD14 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
®
Separator. After removing the column from the magnetic field, the magnetically retained CD14
+
cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD14
+
cells are well-suited for further flow cytometric, functional, or molecular analysis.
Product options: 1

Product information

Size
Order no.
Price

StraightFrom Buffy Coat CD14 MicroBead Kit, human

  • For research use only
for 1
buffy coat
130-114-976
EUR 596,00 

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