StraightFrom® Leukopak® CD8 MicroBead Kit, human

StraightFrom
®
Leukopak
®
CD8 MicroBead Kit
, human

The StraightFrom
®
Leukopak
®
MicroBead Kit was developed for the rapid positive selection of CD8
+
cells directly from Leukopak
®
using the MultiMACS™ Cell24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. Multi-24 Column Block and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a 1/2 Leukopak
®
.

Background information

In blood, the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16
+
NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells.

Detailed separation procedure

A Leukopak
®
sample is labeled with StraightFrom
®
Leukopak
®
CD8 MicroBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
®
Separator. After removing the column from the magnetic field, the magnetically retained CD4
+
T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD8
+
cells are well suited for further flow cytometric, functional, or molecular analysis.

Columns

Multi-24 Column Block
Video

How to isolate immune cells directly from blood

For more information, visit https://bit.ly/2RJiJUd Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1
130-117-019

StraightFrom
®
Leukopak
®
CD8 MicroBead Kit
, human

for 0.5
Leukopak
®
EUR 2.700,00