Cell isolation directly from LRSC

LRSCs arise after routine donor plateletpheresis procedures, during which unwanted leukocytes are filtered out into the LRSC, also known as buffy cone. As LRSCs contain highly concentrated leukocytes in a relatively small volume, they are an ideal source for leukocytes. 

With StraightFrom LRSC MicroBead Kits cells can be directly isolated from an entire or partial LRSC. Together with LS columns (included in the kit) and magnetic separators such as the QuadroMACS™ Separator, leukocytes can be manually enriched. First, the MicroBeads are added directly to the blood sample without any prior cell counting or PBMC isolation. Leukocytes can then be isolated straightforward within minutes without the need for density gradient centrifugation.

Find a product list of all StraightFrom LRSC MicroBead Kits at the end of this page.

The MultiMACS Cell24 Separator Plus is ideally suited to process an entire LRSC in a single run using StraightFrom LRSC MicroBead Kits. 

The 30-minute isolation procedure results in cell populations with high purities and recovery rates. With 12 LS Columns (included in the kit), the instrument allows for time saving processing of large sample volumes, while providing reliability and reproducibility in day-to-day cell separation. 

For large-scale cell separations, the MultiMACS X is the instrument of choice.