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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
StraightFrom® Buffy Coat MicroBeads allow for the magnetic cell isolation directly from buffy coat. Leukocyte subsets such as CD4+, CD8+, CD14+, CD19+, or CD56+ cells can be purified using either MultiMACS™ Instruments or manual separators. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.
CD4+ cells can be isolated straight from a buffy coat using the StraightFrom® Buffy Coat CD4 MicroBead Kit, human and the MultiMACS™ Cell24 Separator Plus.
Separation of a buffy coat sample using the StraightFrom® Buffy Coat CD4 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD4-PE, CD14-APC, as well as CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells were triggered via CD45-VioBlue, cell debris, and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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