Cell isolation directly from buffy coat

As by-products from whole blood donations, buffy coats contain the same amount of leukocytes as the original blood donation, but in higher concentrations. Buffy coats are ideally suited for enrichment of high amounts of leukocyte subset from PBMCs.

With StraightFrom Buffy Coat MicroBead Kits, an entire buffy coat can be processed into target cells in less than 30 minutes.

Together with Whole Blood Columns (included in the kit) and magnetic separators such as the QuadroMACS™ Separator,  leukocytes can be manually enriched. First, the MicroBeads are added directly to the blood sample without any prior cell counting or PBMC isolation. Leukocytes can then be isolated straightforward within minutes without the need for density gradient centrifugation.

Find a product list of all StraightFrom Buffy Coat MicroBead Kits at the end of this page.

The MultiMACS Cell24 Separator Plus is ideally suited to process an entire buffy coat in a single run with StraightFrom Buffy Coat MicroBeads. The 30-minute isolation procedure results in cell populations with high purities and recovery rates. With 12 Whole Blood Columns (included in the kit), the instrument allows for time saving cell isolation when processing large sample volumes, while providing reliability and reproducibility in day-to-day cell separations. 

For large-scale cell separations, the MultiMACS X  is the instrument of choice.