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Mouse mammary gland was dissociated using the Adipose Tissue Dissociation Kit, mouse and rat (# 130-105-808) in combination with the gentleMACS Octo Dissociator. Subsequently, tissue‑resident stem and progenitor cells were pre-enriched using the Tissue Stem Cell Pre-Enrichment Kit, mouse. Cells were fluorescently stained with lineage markers (CD31, CD45, Ter-119 conjugated to APC) and Labeling Check Reagent‑VioBlue and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Stem cell-enriched fraction
Lineage cell-enriched fraction