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Mouse tumor-associated fibroblasts were purified from a mouse B16-F10 tumor (melanoma) using the Tumor-Associated Fibroblast Isolation Kit, mouse. For pre-enrichment an LD Column and a QuadroMACS™ Separator were used, subsequent isolation was performed on a MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with CD45-PE-Vio®
770 and CD90.2-APC, and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Pre-enriched tumor-associated fibroblasts
Isolated tumor-associated fibroblasts
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