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A tumor induced by the B16-F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD4+
TILs were isolated from the single-cell suspension using CD4 (TIL) MicroBeads, optional wash step, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-PE and CD3ε-APC-Vio®
770 and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and DAPI fluorescence.