Naive Pan T Cell Isolation Kit, human

Naive Pan T Cell Isolation Kit, human

The Naive Pan T Cell Isolation Kit was developed for the fast and efficient isolation of untouched naive T cells from human PBMCs.

Detailed separation procedure

For depletion of memory T cells and non-T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD57, CD123, anti-HLA-DR, CD235a (Glycophorin A), and CD244 antibodies. Optionally, biotinylated Anti-TCRγ/δ antibodies can be added for depletion of TCRγ/δ
+
T cells. The cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. CD61 MicroBeads are added for concurrent depletion of platelets.

Downstream applications

Naive pan T cells isolated with the Naive Pan T Cell Isolation Kit can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T cell activation and in functional studies of DCs. Untouched naive pan T cells can also be used for further isolation of specific T cell subsets.

Columns

LS or autoMACS
®
Columns.
Product options: 1

Product information

Size
Order no.
Price

Naive Pan T Cell Isolation Kit, human

  • For research use only
for 1×
10
9
total cells
130-097-095
EUR 599,00 

Related products

6 products available | view all