Naive CD4+ T Cell Isolation Kit II, human

Naive CD4
+
T Cell Isolation Kit II
, human

The Naive CD4
+
T Cell Isolation Kit II was developed for the isolation of untouched naive CD4
+
T cells from human peripheral blood.

Detailed separation procedure

For magnetic depletion of memory CD4
+
T cells and non-CD4
+
T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.

Downstream applications

Naive CD4
+
T cells isolated with the Naive CD4
+
T Cell Isolation Kit II can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T helper cell activation and in functional studies of DCs.
1, 2
Untouched naive CD4
+
T cells can also be used for further isolation of specific T helper cell subsets.
For isolation of naive CD4
+
recent thymic emigrants the CD4
+
Recent Thymic Emigrant Isolation Kit, human is recommended.

Columns

LS, XS, or autoMACS
®
Columns.
Product options: 1

Product information

Size
Order no.
Price

Naive CD4
+
T Cell Isolation Kit II
, human

  • For research use only
for 1×
10
9
total cells
130-094-131
EUR 837,00 

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