CD4 MicroBeads, rat

CD4 MicroBeads, rat

Rat CD4 MicroBeads have been developed for positive selection or depletion of CD4
cells from lymphoid organs, such as spleen and lymph nodes, from peritoneal fluid or single-cell suspensions of lung, liver, kidney, nerve, and heart tissue.

Background information

CD4 is expressed on most thymocytes, T helper cells, CD4
regulatory T cells, as well as on a subset of dendritic cells and, at a lower level, on macrophages and monocytes.

Downstream applications

T cells isolated with CD4 MicroBeads can be used for
in vitro1
analysis, for example, of cytokine expression or antigen specificity. Functionality of the isolated cells has been shown by several adoptive transfer experiments.
Transfer of isolated CD4
and CD8a
T cells into irradiated recipients results in donor-specific acceptance to heart transplants
or type V collagen-induced tolerance to lung allografts
. Furthermore, autoimmunity and induction of tolerance after xenogeneic thymus transplantation was studied by adoptive transfer of T cells separated by MACS® Technology.


For positive selection: MS, LS, XS, or autoMACS
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Lombardi, M. S. et al. (2001) Adjuvant arthritis induces down-regulation of G protein-coupled receptor kinases in the immune system. J. Immunol. 166: 1635-1640
    2. Kataoka, M. et al. (2003) Development of infectious tolerance after donor-specific transfusion and rat heart transplantation. J. Immunol. 171: 204-211
    3. Mizobuchi, T. et al. (2003) Differential expression of Smad7 transcripts identifies the CD4+CD45RChigh regulatory T cells that mediate type V collagen-induced tolerance to lung allografts. J. Immunol. 171: 1140-1147
    4. Yan, Y. et al. (2003) Pathogenesis of autoimmunity after xenogeneic thymus transplantation. J. Immunol. 170: 5936-5946
Product options: 1
for 1×
total cells
EUR 711,00