CD4+ CD62L+ T Cell Isolation Kit II, mouse

CD4
+
CD62L
+
T Cell Isolation Kit II
, mouse

The CD4
+
CD62L
+
T Cell Isolation Kit II was developed for the isolation of mouse CD4
+
CD62L
+
T helper cells from spleen and lymph nodes. The isolation is performed in a two-step procedure.
This product will be discontinued soon. An updated product is available: CD4
+
CD62L
+
T Cell Isolation Kit, mouse, # 130-106-643.

Background information

CD62L (L-selectin) is highly expressed on naive T cells and down-regulated upon activation. It is also expressed on a small subset of memory T helper cells, the central memory T cells, which can be distinguished from naive T helper cells by their high expression of CD44. Furthermore, CD62L is expressed on most thymocytes, naive CD8
+
T cells, B cells, dendritic cells, macrophages, NK cells, neutrophils, eosinophils, regulatory T cells, and TCRγ/δ
+
T cells.

Detailed separation procedure

For isolation of CD4
+
CD62L
+
T helper cells, the non-T helper cells as well as regulatory T cells and γ/δ T cells are depleted by indirect magnetic labeling using a cocktail of lineage-specific biotin-conjugated antibodies against CD8a (Ly-2), CD45R (B220), CD49b (DX5), CD11b (Mac-1), and Ter-119, as well as antibodies against CD25 and TCRγ/δ in combination with Anti-Biotin MicroBeads. Subsequently, CD4
+
CD62L
+
T cells are positively selected from the enriched CD4
+
T helper cell fraction with CD62L (L-selectin) MicroBeads.

Downstream applications

CD4
+
CD62L
+
T helper cells isolated with the CD4
+
CD62L
+
T Cell Isolation Kit II can be used for the analysis of T cell activation by antigen-presenting cells, for studies on cytokine expression and receptor signaling, or for adoptive transfer experiments. Naive T cells, isolated with the CD4
+
CD62L
+
T Cell Isolation Kit, have been used for
in vitro
polarization into Th1 and Th2 cells and were further analyzed for cytokine production after treatment with IL-33.
1
In addition, isolated naive T cells were used in a study about the influence of the golli protein (an alternatively spliced product of the myelin basic protein gene) on the regulation of calcium influx in T cells. Isolated naive T cells were e.g. analyzed by calcium imaging as well as in patch clamp experiments.
2

Columns

For the first magnetic separation (depletion): LS or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD4
+
CD62L
+
T Cell Isolation Kit II
, mouse

  • For research use only
for 1×
10
9
total cells
130-093-227
EUR 771,00 

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