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Technical support - Clinical products
T cells were isolated from a mouse spleen cell suspension using the CD4+
T Cell Isolation Kit, an LS and an MS Column, a MidiMACS™ and a MiniMACS™ Separator. The cells were fluorescently stained with CD4-FITC and CD62L-APC for detection of naive T cells and with CD62L-APC and CD44-PE for detection of central memory T cells. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
Spleen cells before separation
Pre-enriched CD4+ cells after depletion of non-CD4+ T cells
Isolated CD4+ CD62L+ T cells