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regulatory T cells were isolated from human PBMCs by using the CD4+
Regulatory T Cell Isolation Kit II, an LD and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. The cells were fluorescently stained with CD4-FITC, CD25-APC, and CD127-PE, or CD4-FITC, CD127-PE, and Anti-FoxP3-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to CD4 expression, except for the CD25-APC vs. CD4-FITC dot plot.
PBMCs before separation
Pre-enriched CD4+ CD127dim/- cells
Isolated CD4+ CD25+ CD127dim/- regulatory T cells