CD25+ CD49d– Regulatory T Cell Isolation Kit, human

CD25
+
CD49d
Regulatory T Cell Isolation Kit
, human

The CD25
+
CD49d
-
Regulatory T Cell Isolation Kit was developed for the isolation of CD4
+
CD25
+
CD49d
-
regulatory T cells from human PBMCs in a two-step procedure.

Background information

CD49d is the α-chain of the integrin VLA-4 and is present on the majority of pro-inflammatory effector cells but absent on FoxP3
+
cells. Consequently, the depletion of CD49d
+
cells is a useful tool to isolate highly pure populations of CD25
+
CD49d
regulatory T cells.

Detailed separation procedure

The isolation of CD25
+
CD49d
regulatory T cells is performed in a two-step procedure. First, CD8
+
and CD49d
+
cells are magnetically labeled with a cocktail of CD8 and CD49d MicroBeads for depletion. In the second step, CD49d
regulatory T cells are labeled with CD25 MicroBeads and isolated by positive selection from the pre-enriched T cell fraction.
The depletion of CD49d
+
cells removes contaminating CD25
+
effector T cells
1
, thus providing highly pure populations of regulatory T cells.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD25
+
CD49d
Regulatory T Cell Isolation Kit
, human

  • For research use only
for 1×
10
9
total cells
130-094-551
EUR 1.006,00 

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