CD56+ CD16- NK Cell Isolation Kit, human

CD56
+
CD16
-
NK Cell Isolation Kit
, human

The CD56
+
CD16
NK Isolation Kit was developed for the isolation of CD56
bright
CD16
NK cells from PBMCs by sequential sorting. CD56
bright
CD16
NK cells have a high capacity for cytokine production and are thus suggested to have an immunoregulatory role.

Background information

NK cells represent a highly specialized lymphocyte population of the innate immune system, playing an important role in early immune response.
NK cells can be divided into several subsets according to function and phenotype. CD56
+
CD16
NK cells represent the minority of all NK cells in the blood. They lack expression of killer cell immunoglobulin-like receptors (KIRs) and, accordingly, neither show strong cell-mediated cytotoxicity nor antibody-dependent cell-mediated cytotoxicity (ADCC). They are potent producers of cyokines, predominantly IFN-γ, thus linking innate and adaptive immune system.
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Detailed separation procedure

The isolation of CD56
+
CD16
-
NK cells is performed in a two-step procedure. First, non-NK cells and CD56
+
CD16
+
NK cells are labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads and subsequently depleted by separation over a MACS® Column, which is placed in the magnetic field of a MACS Separator. In the second step, the target cells are labeled with CD56 MicroBeads and isolated by positive selection from the pre-enriched cell fraction by using a second column.

Downstream applications

Cells isolated using the CD56
+
CD16
-
NK Cell Isolation Kit can be used for the phenotypical and functional characterization of NK cell subsets, for the analysis of receptor expression, cytokine secretion, or cell-cell interactions.
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Furthermore, cells can be used to study the role of NK cells in diseases or pregnancy.

Columns

For the first magnetic separation (depletion): LS or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD56
+
CD16
-
NK Cell Isolation Kit
, human

  • For research use only
for 2×
10
9
total cells
130-092-661
EUR 976,00 

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