CD140a (PDGFRα) MicroBead Kit, mouse

CD140a (PDGFRα) MicroBead Kit, mouse

The CD140a (PDGFRα) MicroBead Kit was developed for the direct isolation or depletion of CD140a (PDGFRα) positive oligodendrocyte precursors from dissociated mouse brain tissue.

Background information

CD140a (PDGFRα) MicroBeads (PDGFRα: platelet-derived growth factor receptor alpha) have been developed for the separation of mouse cells based on the expression of the CD140a (PDGFRα) antigen. CD140a (PDGFRα) has been reported to be broadly expressed in embryonic tissue, various malignancies and embryonic stem cell-derived cardiomyogenic cells.
Furthermore, CD140a (PDGFRα) is specifically expressed by oligodendrocyte precursor cells, which differentiate into myelinating oligodendrocytes.
CD140a (PDGFRα) MicroBeads were especially optimized for the separation of oligodendrocyte progenitor cells (OPCs) based on the expression of CD140a (PDGFRα). The isolation was tested particularly on dissociated postnatal CD1 mouse brain tissue derived from animals younger than postnatal day eight (P8). In principle, cell isolation is also possible from older mice, but purity of the positive fraction might be lower due to lower frequency of CD140a (PDGFRα) positive cells after tissue dissociation.
For optimal results, the Neural Tissue Dissociation Kit (P) is recommended prior to cell isolation.
  • Selected references

    1. Heldin, C.H. and Westermark, B. (1999)
      Mechanism of action and
      in vivo
      role of platelet-derived growth factor.
      Physiol. Rev. 79: 1283-1316
    2. Takakura, N. et al. (1997) PDGFRα expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal antibody anti-mouse PDGFR alpha antibody APA5. J. Histochem. Cytochem. 45(6): 883-893
    3. Sim, F. J. et al. (2011) CD140a identifies a population of highly myelinogenic migration-competent and efficiently engrafting human oligodendrocyte progenitor cells. Nat. Biotechnol. 29: 934-941
    4. Nishiyama, A. et al. (1996) Co-localization of NG2 proteoglycan and PDGF alpha-receptor on O2A progenitor cells in the developing rat brain. J. Neurosci. Res. 43: 299-314
    5. Nishiyama, A. et al. (2009) Polydendrocytes (NG2 cells): multifunctional cells with lineage plasticity. Nat. Rev. Neurosci. 10: 9-22
Product options: 2
for 5×
total cells
EUR 70,00 
for 1×
total cells
EUR 667,00 

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