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cells (positive fraction) were isolated from day 7 postnatal mouse brain tissue using the Neural Tissue Dissociation Kit (T), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-GLAST (ACSA-1) MicroBead Kit, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Anti-GLAST (ACSA-1)-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Mouse forebrain cells before separation
GLAST (ACSA-1)-negative cells