Indirect CD34 MicroBead Kit, human

Indirect CD34 MicroBead Kit, human

The Indirect CD34 MicroBead Kit was developed for the isolation of CD34
+
precursor cells, using a two-step procedure involving indirect magnetic labeling from
  • peripheral blood
  • bone marrow
  • leukapheresis product
  • cord blood
  • differentiated-ES and iPS cells
Even faster, one-step isolation of CD34
+
cells can be achieved with the CD34 MicroBead Kit.

Background information

CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts
1,2
, endothelial progenitor cells
1,3-5
, and mature endothelial cells
4
.

Detailed separation procedure

First, cells are labeled with the hapten-modified CD34 primary antibody QBEND/10
6
. In a second step, antibody-labeled cells are magnetically labeled with Anti-Hapten MicroBeads.
Control staining of cells isolated with the Indirect CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

  • Transplantation of CD34+ cells into humanized mouse models
  • Expansion and differentiation in culture
  • Reprogramming of CD34+ HSCs into iPS cells
  • Differentiation of ES or iPS cell-derived CD34+ -derived precursors into endothelial or hematopoietic lineages
  • Evaluation of differentiation potential in CFU assays
  • Characterization of surface marker expression by flow cytometry.

Columns

MS, LS, XS, or autoMACS
®
Columns.
  • Selected References

    1. Wang, Z. Z. et al. (2007) Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo. Nat. Biotechnol. 25: 317-318
    2. Loges, S. et al. (2004) Identification of the adult human hemangioblast. Stem Cells Dev. 13: 229-242
    3. Peichev, M. et al. (2000)
      Expression of VEGFR-2 and AC133 by circulating human CD34
      +
      cells identifies a population of functional endothelial precursors.
      Blood 95: 952-958
    4. Rafii, S. and Lyden, D. (2003) Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat. Med. 9: 702-712
    5. Valgimigli, M. et al. (2004)
      CD34
      +
      and endothelial progenitor cells in patients with various degrees of congestive heart failure.
      Circulation 110: 1209-1212
    6. Lanza, F. et al. (2001) Structural and functional features of the CD34 antigen: an update. J. Biol. Regul. Homeost. Agents 15: 1-13
Product options: 1

Product information

Size
Order no.
Price

Indirect CD34 MicroBead Kit, human

  • For research use only
for 2×
10
9
total cells
130-046-701
EUR 677,00