CD34 MicroBead Kit, human

CD34 MicroBead Kit, human

The CD34 MicroBead Kit has been developed for the positive selection of CD34
+
hematopoietic stem and progenitor cells in a single step. CD34
+
cells can be isolated from:
  • peripheral blood (PBMCs)
  • bone marrow1
  • leukapheresis product2
  • cord blood3
  • differentiated ES and iPS cell lines4,5 .

Background information

CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts
5,6
, endothelial progenitor cells
5,7-9
, and mature endothelial cells
8
.
Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.

Downstream applications

Cells isolated with the CD34 MicroBead Kit are suitable for various downstream applications, including:
  • flow cytometric analysis
  • expansion in culture
  • in vitro differentiation5
  • reprogramming3
  • retroviral transduction10
  • gene expression analyses
  • transplantation into humanized mice

Columns

MS, LS, XS, or autoMACS
®
Columns.
  • Selected References

    1. Brault, J. et al. (2014)
      Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
      ex vivo
      models of X
      0
      -linked, AR22
      0
      - and AR47
      0
      - chronic granulomatous diseases.
      Biores Open Access 3(6): 311-326
    2. Schreiber, T. D. et al. (2009) The integrin alpha9beta1 on hematopoietic stem and progenitor cells: involvement in cell adhesion, proliferation and differentiation. Haematologica 94: 1493-1501
    3. Jing et al. (2010) CD49d blockade by natalizumab in patients with multiple sclerosis affects steady-state hematopoiesis and mobilizes progenitors with a distinct phenotype and function. Bone Marrow Transplant. 45: 1489-1496
    4. Ban, H. et al. (2011) Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc. Natl. Acad. Sci. U.S.A. 108: 14234-9
    5. Bandi, S. and Akkina, R. (2008) Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection. AIDS Res. Ther. 5: 1
    6. Wang, Z. Z. et al. (2007) Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo. Nat. Biotechnol. 25: 317-318
    7. Loges, S. et al. (2004) Identification of the adult human hemangioblast. Stem Cells Dev. 13: 229-242
    8. Peichev, M. et al. (2000)
      Expression of VEGFR-2 and AC133 by circulating human CD34
      +
      cells identifies a population of functional endothelial precursors.
      Blood 95: 952-958
    9. Rafii, S. and Lyden, D. (2003) Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat. Med. 9: 702-712
    10. Valgimigli, M. et al. (2004)
      CD34
      +
      and endothelial progenitor cells in patients with various degrees of congestive heart failure.
      Circulation 110: 1209-1212
    11. Chen et al. (2011) Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in mice. Blood 117: 1530-1539
  • Customer reports and application notes

Product options: 3

Product information

Size
Order no.
Price

CD34 MicroBead Kit, human

  • For research use only
for 2×
10
9
total cells
130-046-702
EUR 691,00 

CD34 MicroBead Kit, human

  • For research use only
for 1×
10
10
total cells
130-046-703
EUR 2.403,00 

CD34 MicroBead Kit, human - lyophilized

  • For research use only
for 2×
10
9
total cells
130-097-047
EUR 693,00