CD34+ CD38- Cell Isolation Kit, human

CD34
+
CD38
-
Cell Isolation Kit
, human

The CD34
+
CD38
Cell Isolation Kit, human has been developed to isolate a CD38
subset of CD34
+
cells, i.e., primitive hematopoietic stem and progenitor cells, from cord blood, bone marrow, or apheresis.

Background information

The CD34
+
CD38
Cell Isolation Kit has been developed for the isolation of the more primitive CD38
subpopulation of CD34
+
cells. CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts, endothelial progenitor cells, and mature endothelial cells. CD38 is absent on most primitive CD34
+
stem cells and upregulated on CD34
+
cells upon lymphocyte commitment. CD34
+
CD38
-
cells are isolated in a two-step isolation protocol. First, the CD34
+
cells are positively enriched using CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34
+
are then labeled with CD38 MicroBeads to deplete CD38
+
cells in a second separation step.

Downstream applications

  • CD34+ CD38 cell expansion in culture
  • Lentiviral transduction
  • Molecular analysis
  • In vitro differentiation of CD34+ CD38 cells
  • Generation of humanized mice

Columns

LS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD34
+
CD38
-
Cell Isolation Kit
, human

  • For research use only
for 2×
10
9
total cells
130-114-822
EUR 723,00 

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