Diamond Basophil Isolation Kit, human

Diamond Basophil Isolation Kit, human

The Diamond Basophil Isolation Kit has been developed for the two-step isolation of basophilic granulocytes from human PBMCs.

Background information

Basophilic granulocytes, also known as basophils, are a rare population of leukocytes with frequencies below 1% in peripheral blood. First described by Paul Ehrlich in 1879, basophils have long been considered as circulating mast cells. They express high-affinity immunoglobulin (Ig) E receptors (FcεRI). Upon receptor cross-linking by binding of antigen-IgE complexes, basophils degranulate and thereby release inflammatory mediators such as histamine and leukotriene. Thus, basophils play a major role in allergy, asthma, and immunity.
1-4

Detailed separation procedure

First, non-basophils are magnetically labeled for depletion over a MACS Column. The pre-enriched non-labeled basophils in the flow-through are then positively selected via CD123 MicroBeads.

Downstream applications

  • Studies on signal requirements for basophil activation, induction of mediator synthesis and release etc.
  • Studies on the involvement of basophils in hypersensitivity reactions
  • Studies on cytokine expression by basophils

Columns

For the first magnetic separation (depletion): LS or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
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Product information

Size
Order no.
Price

Diamond Basophil Isolation Kit, human

  • For research use only
for 2×
10
9
total cells
130-094-434
EUR 1.103,00 

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