Anti-TRA-1-60 MicroBeads, human

Anti-TRA-1-60 MicroBeads, human

The direct Anti-TRA-1-60 MicroBeads and the indirect Anti-TRA-1-60 MicroBead Kit have been developed for the positive selection of human pluripotent stem cells based on the expression of the TRA-1-60 antigen.
The direct Anti-TRA-1-60 MicroBeads offer a 10 min isolation procedure, minimizing cell handling time.

Background information

The Anti-TRA-1-60 monoclonal antibody reacts with a pluripotent stem cell–specific antigen expressed on undifferentiated human embryonic stem (ES) cells, induced pluripotent (iPS) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells.
2,3
The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The Anti-TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers including breast cancer and prostate cancer.

Applications

  • Positive selection of undifferentiated TRA-1-60+ pluripotent stem cells, for example, human ES and iPS cells.
  • Efficient enrichment of iPS cells during or after reprogramming1,5 .

Columns

MS or autoMACS
®
Columns for use with Anti-TRA-1-60 MicroBeads.
LS or autoMACS Columns for use with the Anti-TRA-1-60 MicroBead Kit.
Product options: 2

Product information

Size
Order no.
Price

Anti-TRA-1-60 MicroBeads, human

  • For research use only
for
up to
10
8
total cells
130-100-832
EUR 625,00 

Anti-TRA-1-60 MicroBead Kit, human

  • For research use only
for 2×
10
8
total cells
130-095-816
EUR 604,00 

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