Pan-DC Enrichment Kit, human

Pan-DC Enrichment Kit, human

The Pan-DC Enrichment Kit is a fast pre-enrichment kit for untouched isolation of plasmacytoid and myeloid dendritic cells from PBMCs.

Detailed separation procedure

Using the Pan-DC Enrichment Kit, dendritic cells (MDC1s, MDC2s, and PDCs) are isolated very fast in a single step by depletion of non-target cells (negative selection). Non-target cells are labeled with a cocktail of biotin-conjugated monoclonal antibodies and Anti-Biotin MicroBeads. Additionally, they are directly magnetically labeled with a cocktail of MicroBead-conjugated antibodies against antigens that are not expressed by dendritic cells. The magnetically labeled non-target cells are depleted by retaining them within a MACS Column in the magnetic field of a MACS Separator while the unlabeled DCs run through.

Downstream applications

Dendritic cells pre-enriched with the Pan-DC Enrichment Kit can be used for:
  • Analysis of antigen uptake, processing, and presentation
  • Stimulation of primary T cell responses by antigen-pulsed dendritic cells
  • Analysis of gene expression in blood dendritic cells
  • Elucidation of specific signal transduction pathways

Columns

LS, XS, or autoMACS
®
Columns.
  • Selected References

    1. O’Doherty, U. et al. (1994) Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature. Immunology 82: 487-493
    2. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    3. Penna, G. et al. (2001) Cutting Edge: Selective usage of chemokine receptors by plasmacytoid dendritic cells.. J. Immunol. 167: 1862-1866
    4. Penna, G. et al. (2002) Cutting edge: Differential chemokine production by myeloid and plasmacytoid dendritic cells. J. Immunol. 169: 6673-6676
    5. Krug, A. et al. (2001) Toll-like receptor reveals CpG DNA as a unique microbial vstimulus for plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12. Eur. J. Immunol. 31: 3026-3037
Product options: 1

Product information

Size
Order no.
Price

Pan-DC Enrichment Kit, human

  • For research use only
for 2×
10
9
total cells
130-100-777
EUR 640,00