Diamond Plasmacytoid Dendritic Cell Isolation Kit II, human

Diamond Plasmacytoid Dendritic Cell Isolation Kit II, human

The Diamond Plasmacytoid Dendritic Cell Isolation Kit II yields PDC populations with excellent purities. The kit involves a two-step procedure, consisting of depletion of non-PDCs and positive selection using MicroBeads against the PDC-specific antigen CD304 (BDCA-4/Neuropilin-1). This kit has been developed based on the first-generation kit (# 130-092-402) for even better performance and a shorter protocol.

Background information

The isolated PDCs are CD303 (BDCA-2)
, CD304 (BDCA-4/Neuropilin-1)
, CD123
, CD4
, CD45RA
, CD141 (BDCA-3)
, CD1c (BDCA-1)
, and CD2
. The cells lack expression of lineage markers (CD3, CD14, CD16, CD19, CD20, CD56) and express neither myeloid markers, such as CD13 and CD33, nor Fc receptors, such as CD16, CD64, or FcεRI.
Binding of the antibody to CD304 (BDCA-4/Neuropilin-1) does not have a significant effect on IFN type I production in PDCs. This is in contrast to binding of antibodies to the other PDC-specific antigen, CD303 (BDCA‑2).
1, 2

Detailed separation procedure

For the depletion step, all non-PDCs are magnetically labeled by using cocktails containing specific MicroBeads, biotin-conjugated antibodies, and Anti-Biotin MicroBeads. For the subsequent positive selection step, the enriched PDCs are magnetically labeled with CD304 (BDCA-4/Neuropilin-1) Diamond MicroBeads.

Downstream applications

The Diamond Plasmacytoid Dendritic Cell Isolation Kit II was developed for the isolation of PDCs from PBMCs with maximal purity. It is particularly useful if a PDC population with exceptionally high purity is needed as starting material, e.g. for gene expression profiling or protein purification.


For the first magnetic separation (depletion): LD or autoMACS
Columns. For the second magnetic separation (positive selection): MS, LS, or autoMACS Columns.
  • Selected references

    1. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 63: 1133-1148
    2. Colonna, M. et al. (2004) Plasmacytoid dendritic cells in immunity. Nat. Immunol. 5: 1219-1226
Product options: 1
for 2×
total cells
EUR 1.105,00 

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