CD4+ Dendritic Cell Isolation Kit, mouse

CD4
+
Dendritic Cell Isolation Kit
, mouse

The CD4
+
Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4
+
DCs from single-cell suspensions of splenocytes.

Background information

CD4
+
DCs coexpress CD11c, CD11b, MHC class II, CD40, CD80, CD86 and are negative for CD8 and CD205. They represent the major population of DCs in spleen and comprise only a small population in peripheral lymph nodes (56% and 4% of all CD11c
+
DCs, respectively). The isolation of CD4
+
DCs is performed in two steps.

Detailed separation procedure

First, DCs are enriched by depletion of T cells, NK cells, B cells, and granulocytes with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Subsequently, CD4
+
DCs are positively selected from the enriched DC fraction with CD4 MicroBeads.

Downstream applications

CD4
+
DCs isolated using the CD4
+
Dendritic Cell Isolation Kit are suitable to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T cell polarization by CD4
+
DCs.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD4
+
Dendritic Cell Isolation Kit
, mouse

  • For research use only
for 2×
10
9
total cells
130-091-262
EUR 771,00 

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