CD304 (BDCA-4/Neuropilin-1) MicroBead Kit, human

CD304 (BDCA-4/Neuropilin-1) MicroBead Kit, human

The CD304 (BDCA-4/Neuropilin-1) MicroBead Kit has been developed for the enrichment of plasmacytoid dendritic cells (PDCs) from PBMCs by positive selection.

Background information

CD304 (BDCA-4/Neuropilin-1)
is specifically expressed by PDCs in human blood
, bone marrow
, and cord blood
. Exclusive expression of CD304 (BDCA-4/Neuropilin-1) on PDCs allows their direct isolation. In blood and bone marrow, CD304 (BDCA-4/Neuropilin-1)
PDCs are CD4
, CD45RA
, CD303 (BDCA-2)
, CD141 (BDCA-3)
, CD1c (BDCA-1)
, and CD2
. They lack expression of lineage markers (CD3, CD14, CD16, CD19, CD20, CD56) and neither express myeloid markers such as CD13 and CD33, nor Fc receptors such as CD32, CD64, or FcεRI. Their lymphoid origin is indicated by their plasmacytoid morphology and the expression of the pre-T cell receptor α-chain. Freshly isolated CD1c (BDCA-1)
or CD141 (BDCA-3)
myeloid blood dendritic cells and monocytes do not express CD304 (BDCA-4/Neuropilin-1), but expression of CD304 (BDCA-4/Neuropilin-1) is induced on myeloid blood dendritic cells and monocytes upon culturing.
1, 6
In inflamed tonsils, CD304 (BDCA-4/Neuropilin-1) expression is, apart from PDCs, also detected on some other cells, primarily follicular B helper memory T cells.
CD304 (BDCA-4) was shown to be identical to neuropilin-1 (NP-1).
Neuropilin-1 has formerly been discovered to be expressed on numerous nonhematopoietic cell types including neurons, endothelial and tumor cells.
Unlike binding of antibodies to CD303 (BDCA-2), binding of antibodies to CD304 (BDCA-4/Neuropilin-1) does not have a substantial effect on IFN type I production in PDCs after induction by, e.g., influenza virus.

Downstream applications

Isolated CD304 (BDCA-4/Neuropilin-1)
PDCs were used, for example, to examine expression of Toll-like receptors
4, 7, 8, 12
, chemokine receptors
3, 8, 10
, or antigens, e.g., EMR
and for studies on dendritic cell activation
, migration
, cytokine production
4, 6, 8
, and T cell polarization
4, 6, 13
. Functional and phenotypical analysis was performed, e.g., of PDCs isolated from PMBCs and synovial fluid from psoriatic arthritis and rheumotoid arthritis patients


MS, LS, or autoMACS
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