CD304 (BDCA-4/Neuropilin-1) MicroBead Kit, human

CD304 (BDCA-4/Neuropilin-1) MicroBead Kit, human

The CD304 (BDCA-4/Neuropilin-1) MicroBead Kit has been developed for the enrichment of plasmacytoid dendritic cells (PDCs) from PBMCs by positive selection.

Background information

CD304 (BDCA-4/Neuropilin-1)
is specifically expressed by PDCs in human blood
, bone marrow
, and cord blood
. Exclusive expression of CD304 (BDCA-4/Neuropilin-1) on PDCs allows their direct isolation. In blood and bone marrow, CD304 (BDCA-4/Neuropilin-1)
PDCs are CD4
, CD45RA
, CD303 (BDCA-2)
, CD141 (BDCA-3)
, CD1c (BDCA-1)
, and CD2
. They lack expression of lineage markers (CD3, CD14, CD16, CD19, CD20, CD56) and neither express myeloid markers such as CD13 and CD33, nor Fc receptors such as CD32, CD64, or FcεRI. Their lymphoid origin is indicated by their plasmacytoid morphology and the expression of the pre-T cell receptor α-chain. Freshly isolated CD1c (BDCA-1)
or CD141 (BDCA-3)
myeloid blood dendritic cells and monocytes do not express CD304 (BDCA-4/Neuropilin-1), but expression of CD304 (BDCA-4/Neuropilin-1) is induced on myeloid blood dendritic cells and monocytes upon culturing.
1, 6
In inflamed tonsils, CD304 (BDCA-4/Neuropilin-1) expression is, apart from PDCs, also detected on some other cells, primarily follicular B helper memory T cells.
CD304 (BDCA-4) was shown to be identical to neuropilin-1 (NP-1).
Neuropilin-1 has formerly been discovered to be expressed on numerous nonhematopoietic cell types including neurons, endothelial and tumor cells.
Unlike binding of antibodies to CD303 (BDCA-2), binding of antibodies to CD304 (BDCA-4/Neuropilin-1) does not have a substantial effect on IFN type I production in PDCs after induction by, e.g., influenza virus.

Downstream applications

Isolated CD304 (BDCA-4/Neuropilin-1)
PDCs were used, for example, to examine expression of Toll-like receptors
4, 7, 8, 12
, chemokine receptors
3, 8, 10
, or antigens, e.g., EMR
and for studies on dendritic cell activation
, migration
, cytokine production
4, 6, 8
, and T cell polarization
4, 6, 13
. Functional and phenotypical analysis was performed, e.g., of PDCs isolated from PMBCs and synovial fluid from psoriatic arthritis and rheumotoid arthritis patients


MS, LS, or autoMACS
  • Selected references

    1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    2. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
    3. Penna, G. et al. (2001) Cutting Edge: Selective usage of chemokine receptors by plasmacytoid dendritic cells.. J. Immunol. 167: 1862-1866
    4. Krug et al. (2001) Toll-like receptor expression reveals CpG DNA as a unique microbial stimulus for plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12. Eur. J. Immunol. 31: 3026-3037
    5. Dzionek, A. et al. (2001) BDCA-2, a novel plasmacytoid dendritic cell–specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon α/β induction. J. Exp. Med. 194: 1823-1834
    6. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 63: 1133-1148
    7. Hornung, V. et al. (2002) Quantitative expression of toll-like receptor 1-10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides. J. Immunol. 168: 4531-4537
    8. Jarrossay et al. (2001) Specialization and complementarity in microbial molecule recognition by human myeloid and plasmacytoid dendritic cells. Eur. J. Immunol. 31: 3388-3393
    9. Kwakkenbos, M. J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells. J. Leukoc. Biol. 71(5): 854-862
    10. Penna, G. et al. (2002) Cutting edge: differential chemokine production by myeloid and plasmacytoid dendritic cells. J. Immunol. 169: 6673-6676
    11. De Wit, D. et al. (2004) Blood plasmacytoid dendritic cell responses to CpG oligonucleotides are impaired in human newbornes. Blood 103: 1030-1032
    12. Matsumoto, M. et al. (2003) Subcellular localization of Toll-like receptor 3 in human dendritic cells. J. Immunol. 171: 3154-3162
    13. Kaser, A. et al. (2004) Interleukin-18 attracts plasmacytoid dendritic cells (DC2s) and promotes Tʜ1 induction by DC2s through IL-18 receptor expression. Blood 103: 648-655
    14. Colonna, M. et al. (2004) Plasmacytoid dendritic cells in immunity. Nat. Immunol. 5: 1219-1226
    15. Jongbloed, S. L. et al. (2006) Enumeration and phenotypical analysis of distinct dendritic cell subsets in psoriatic arthritis and rheumatoid arthritis. Arthritis Res. Ther. 8: R15
  • Scientific posters

Product options: 2
for 2×
total cells
EUR 1.028,00 
for 2×
total cells
EUR 997,00 

Related products

5 products available | view all