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Isolation of CD1c (BDCA-1)+
dendritic cells from rhesus monkey PBMCs using the CD1c (BDCA-1)+
Dendritic Cell Isolation Kit. Cells were incubated with CD1c (BDCA-1)-PE and subsequently magnetically labeled with CD20 MicroBeads. B cells were depleted using an LD Column. The B cell depleted fraction was magnetically labeled with Anti-PE MicroBeads and separated over an MS Column. Cell samples were stained with CD20-FITC and CD14-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
PBMCs before separation
B cell–depleted flow-through fraction
Counter-staining of B cell depleted flow-through fraction with CD14-APC
blood dendritic cells after enrichment