CD1c (BDCA-1)+ Dendritic Cell Isolation Kit, non-human primate

CD1c (BDCA-1)
+
Dendritic Cell Isolation Kit
, non-human primate

The CD1c (BDCA-1)
+
Dendritic Cell Isolation Kit includes FcR Blocking Reagent, CD1c (BDCA-1)-PE, Anti-PE MicroBeads for the enrichment of CD1c (BDCA-1)
+
blood dendritic cells. CD20 MicroBeads are included for depletion of B cells prior to DC isolation.

Background information

Human as well as rhesus monkey dendritic cells represent a heterogenous population composed of several subsets. Myeloid and plasmacytoid DCs, differentiated by their expression of CD11c and CD123, respectively, were further characterized by means of new markers against human blood dendritic cell antigens (BDCA). Two human myeloid dendritic cell populations have been described: CD11c
high
CD123
low
CD1c (BDCA1)
+
DCs and CD11c
dim
CD123
CD141 (BDCA-3)
+
DCs. Plasmacytoid CD11c
CD123
+
dendritic cells are characterized by the expression of CD303 (BDCA-2) or CD304 (BDCA-4).
1
In rhesus monkey, CD1c is expressed on myeloid CD11c
+
CD123
dendritic cells
2
as well as on a subset of B cells. The CD1c and CD20 antibodies have been tested to cross-react with antigens of the cynomolgus monkey (
Macaca fascicularis
).

Applications

The CD1c (BDCA-1)
+
Dendritic Cell Isolation Kit can be used for the separation of CD1c
+
dendritic cells from rhesus monkey (
Macaca mulatta
) PBMCs.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS, LS, or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD1c (BDCA-1)
+
Dendritic Cell Isolation Kit
, non-human primate

  • For research use only
for 1×
10
9
total cells
130-091-878
EUR 944,00 

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