CD1c (BDCA-1)+ Dendritic Cell Isolation Kit, human

CD1c (BDCA-1)
+
Dendritic Cell Isolation Kit
, human

The CD1c (BDCA-1)
+
Dendritic Cell Isolation Kit has been developed for the isolation of CD1c (BDCA-1)
+
myeloid dendritic cells (MDCs; type-1 MDCs or MDC1s) from PBMCs.

Background information

The CD1c (BDCA-1) antigen is specifically expressed on dendritic cells, which are CD11c
high
CD123
low
and represent the major subset of MDCs in human blood (about 0.6 % of all PBMCs).
1–7
CD1c (BDCA-1)
+
MDCs show a monocytoid morphology and express myeloid markers such as CD13 and CD33 as well as Fc receptors such as CD32, CD64, and FceRI. Furthermore, they were determined to be CD4
+
, Lin (CD3, CD16, CD19, CD20, CD56)
, CD2
+
, CD45RO
+
, CD141 (BDCA-3)
low
, CD303 (BDCA-2)
, and CD304 (BDCA-4/Neuropilin-1)
.
1
A minor proportion of CD1c (BDCA-1)
+
MDCs expresses CD14 and CD11b. CD1c (BDCA-1) is also found on CD1a
+
dendritic cells generated
ex vivo
from monocytes or hematopoietic precursor cells. In blood, apart from MDCs, a subset of B cells also expresses CD1c (BDCA-1). Therefore, the CD1c (BDCA-1)
+
Dendritic Cell Isolation Kit includes CD19 MicroBeads for depletion of B cells prior to the enrichment of CD1c (BDCA-1)
+
MDCs. In order to discriminate the CD1c (BDCA-1)
+
from CD1c (BDCA-1)
CD141 (BDCA-3)
++
MDCs, the former have been designated type-1 MDCs (MDC1s).
The CD1c antigen is a member of the CD1 family of proteins that are structurally related to MHC class I proteins and mediate the presentation of non-peptide antigens towards T cells.
14

Detailed separation procedure

First, B cells are depleted with CD19 MicroBeads. Then, MDC1s are enriched by magnetic labeling with biotin-conjugated CD1c antibodies and Anti-Biotin MicroBeads.

Downstream applications

Isolated CD1c (BDCA-1)
+
MDCs were used to examine the expression of Toll-like receptors
4, 5, 12
, chemokine receptors
3, 5, 8
, or new antigens, e.g., DCAL-16 and EMR27. Further, they were isolated for studies on dendritic cell activation
4
, migration
3
, cytokine production
4, 5, 10
, and T cell polarization
4
, particularly in comparison with monocyte-derived dendritic cells
9
. The CD1c (BDCA-1)
+
Dendritic Cell Isolation Kit was also used for isolation of MDCs from mice engrafted with human CD34
+
hematopoietic progenitor cells
11
, for gene clustering of dendritic cell subsets in blood and tonsils
15
, for phenotypical analysis of MDCs in PBMCs and synovial fluid of psoriatic arthritis and rheumatoid arthritis patients
16
, or as an alternative to CD1a MicroBeads for the isolation of epidermal Langerhans cells
13
.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS, LS, or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD1c (BDCA-1)
+
Dendritic Cell Isolation Kit
, human

  • For research use only
for 2×
10
9
total cells
130-090-506
EUR 988,00 

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