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Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4+
cells were enriched per 106
T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4+
T cell was detected per 106
T cells after enrichment.
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