CD27 MicroBeads, human

CD27 MicroBeads, human

CD27 MicroBeads were developed for the positive selection or depletion of CD27
+
cells from human PBMCs or lymphoid tissues. CD27 is differentially expressed on B cells, T cells, and NK cells.

Background information

CD27 is a member of the tumor necrosis factor receptor family, which among peripheral blood B cells is present on memory B cells
1
and plasma cells but not on naive B cells. In human tonsils, expression of the CD27 antigen is found on memory B cells, germinal center B cells, subepithelial marginal zone B cells, and plasma cells. CD27 is further expressed on specific T cell subsets and NK cell subsets.

Applications

T cells can be pre-selected using the CD8
+
T Cell Isolation Kit or the CD4
+
T Cell Isolation Kit and subsequently separated according to CD27 expression.
For the isolation of human memory B cells, we recommend the Memory B Cell Isolation Kit.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected References

    1. van Lier, R. A. et al. (1987) Tissue distribution and biochemical and functional properties of Tp55 (CD27), a novel T cell differentiation antigen. J. Immunol. 139: 1589-1596
    2. Bigler, R. D. et al. (1988) S152 (CD27). A modulating disulfide-linked T cell activation antigen. J. Immunol. 139: 21-28
    3. Klein, U. et al. (1998)
      Human immunoglobulin (Ig)M
      +
      IgD
      +
      peripheral blood B cells expressing the CD27 cell surface antigen carry somatically mutated variable region genes: CD27 as a general marker for somatically mutated (memory) B cells.
      J. Exp. Med. 188: 1679-1689
    4. Tangye, S. G. et al. (1998) Identification of functional human splenic memory B cells by expression of CD148 and CD27. J. Exp. Med. 188: 1691-1703
    5. Hintzen, R. Q. et al. (1993) Regulation of CD27 expression on subsets of mature T-lymphocytes. J. Immunol. 151: 2426-2435
    6. Elson, L. H. et al. (1995)
      Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4
      +
      CD27
      lymphocyte subpopulation.
      J. Immunol. 154: 4294-4301
    7. Hintzen, R. Q. et al. (1994) Characterization of the human CD27 ligand, a novel member of the TNF gene family. J. Immunol. 152: 1762-1773
    8. Hamann, D. et al. (1997)
      Phenotypic and functional separation of memory and effector human CD8
      +
      T cells.
      J. Exp. Med. 186: 1407-1418
    9. De Milito, A. et al. (2004) Mechanisms of hypergammaglobulinemia and impaired antigen-specific humoral immunity in HIV-1 infection. Blood 103: 2180-2186
Product options: 1

Product information

Size
Order no.
Price

CD27 MicroBeads, human

  • For research use only
for 1×
10
9
total cells
130-051-601
EUR 572,00