R848 (Resiquimod)

R848 (Resiquimod)

TLR7 and TLR8 are members of the toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns
1,2
. TLR7 and TLR8 recognize virally derived double- or singlestranded RNA as well as small molecules like R848 (Resiquimod), both leading to a strong Myd88-dependent activation of immune cell
3,4
.

Background information

TLR7 and TLR8 are expressed on human cells with a distinct expression pattern. In human cells, plasmacytoid dendritic cell (pDCs) and B cells express predominantly TLR7, whereas TLR8 is mainly expressed on cells of the myeloid lineage, such as monocytes, macrophages, and myeloid dendritic cells (mDCs). In mice, TLR7 is also expressed on mDCs and monocytes whereas murine TLR8 is functionally impaired
1,2
. TLR7 and TLR8 recognize virally derived double- or singlestranded RNA as well as small molecules like R848 (Resiquimod), both leading to a strong Myd88-dependent activation of immune cells
3,4
. R848 (Resiquimod) is an imidazoquinoline compound, which possesses antiviral and antitumoral activity
3,4
. R848 (Resiquimod) is poorly soluble in water. Therefore, R848 (Resiquimod) is provided as its hydrochloride analog, which improves the solubility of R848 (Resiquimod) in aqueous solution without affecting potency.

Applications

TLR7 and TLR8 agonists can be used for the activation of immune cells, such as human peripheral blood mononuclear cells (PBMCs), murine splenocytes, or isolated immune cells, e.g., B cells, pDCs, mDCs, and monocytes.
  • Selected References

    1. Blasius, A. L. and Beutler, B. (2010) Intracellular toll-like receptors. Immunity 32(3): 305-315
    2. Kawai, T. and Akira, S. (2010) The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat. Immunol. 11(5): 373-384
    3. Hemmi, H. et al. (2002) Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway. Nat. Immunol. 3(2): 196-200
    4. Jurk, M. et al. (2002) Human TLR7 or TLR8 independently confer responsiveness to the antiviral compound R-848. Nat. Immunol. 3(6): 499
    5. Coch, C. et al. (2013)
      A human
      in vitro
      whole blood assay to predict the systemic cytokine response to therapeutic oligonucleotides including siRNA.
      PLoS One 8(8): e71057
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Product information

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R848 (Resiquimod)

  • For research use only
1 mg
130-109-376
EUR 200,00