T Cell Activation/Expansion Kit, human

T Cell Activation/Expansion Kit, human

The T Cell Activation/Expansion Kit was developed to activate and expand human T cells. The kit consists of Anti-Biotin MACSiBead Particles and biotinylated antibodies against human CD2, CD3, and CD28. Anti-Biotin MACSiBead Particles loaded with the biotinylated antibodies are used to mimic antigen-presenting cells and activate resting T cells from PBMCs as well as purified T cells. T cell expansion is achieved by culturing and reactivation at day 14 of culture.

Downstream applications

Activated T cells can be used for any downstream processing, such as cytokine analysis
or immunoprecipitation, and cells can also be transfected with high efficiency. The kit is suitable for the expansion of antigen-specific T cell lines and clones. It is also suitable for the activation of human CD3
NKT cells.
  • Selected references

    1. Lécureuil et al. (2007) Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Blood 109: 3649-3657
    2. Acosta-Rodriguez, E. V. et al. (2007) Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol. 8: 942-948
    3. Sattler et al. (2009) Cytokine-induced human IFN-gamma-secreting effector-memory Th cells in chronic autoimmune inflammation. Blood 113: 1948-1956
    4. Diveu, C. et al. (2009) IL-27 blocks RORc expression to inhibit lineage commitment of Th17 cells. J. Immunol. 182: 5748-5756
    5. Camargo, J. F. et al. (2009) CCR5 expression levels influence NFAT translocation, IL-2 production, and subsequent signaling events during T lymphocyte activation. J. Immunol. 182: 171-182
    6. Duraisingham, S. S. et al. (2009) TLR-stimulated CD34 stem cell-derived human skin-like and monocyte-derived dendritic cells fail to induce Tʜ17 polarization of naive T cells but do stimulate Tʜ1 and Tʜ17 memory responses. J. Immunol. 183: 2242-2251
    7. Wieckowski, E. U. et al. (2009)
      Tumor-derived microvesicles promote regulatory T cell expansion and induce apoptosis in tumor-reactive activated CD8
      T lymphocytes.
      J. Immunol. 183: 3720-3730
Product options: 1
EUR 844,00 

Related products

7 products available | view all