PepTivator NY-ESO-1, human

PepTivator NY-ESO-1, human

PepTivator
®
NY-ESO-1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human NY-ESO-1 protein (UniProt ID: P78358).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
Quantitative, phenotypical, or functional analysis of NY-ESO-1–specific T cell immunity can provide important information on the natural course of immune responses.

Background information

NY-ESO-1 belongs to the cancer-testis family of antigens. It is highly expressed in male germ cells but generally not in other normal tissues. Aberrant expression is found in a variable proportion of a wide range of different cancer types. Patients with advanced NY-ESO-1-expressing tumors frequently develop humoral and cellular immunity to NY-ESO-11. NY-ESO is an attractive antigen to monitor anti-tumor immune responses and is used for immunotherapeutic interventions.

Downstream applications

The
in vitro
stimulation of NY-ESO-1–specific CD4
+
and CD8
+
T cells with the PepTivator NY-ESO-1 peptide pool causes the secretion of effector cytokines and the up-regulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of NY-ESO-1–specific T cells.
  • Detection and analysis of specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies
  • Isolation of viable tyrosinase–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of tyrosinase–specific CD4+ and CD8+ effector/memory T cells for research on immunotherapy and vaccination
  • Pulsing of antigen-presenting cells.
Product options: 2

Product information

Size
Order no.
Price

PepTivator NY-ESO-1 - premium grade, human

  • For research use only
for
stimulation of
10
8
cells
130-095-380
EUR 213,00 

PepTivator NY-ESO-1 - premium grade, human

  • For research use only
for
stimulation of
10
9
cells
130-095-381
EUR 1.246,00 

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