StemMACS™ Lin28 mRNA, human

StemMACS™ Lin28 mRNA, human

StemMACS™ Lin28 mRNA encodes the human RNA-binding protein Lin28 and is designed for transient transfection.

Applications

  • Modulation of pluripotency
  • Reprogramming

Background information

The RNA binding protein Lin28 is a conserved regulator of cell fate. In vertebrates, Lin28 promotes pluripotency and controls differentiation in diverse tissues. Lin28 can directly inhibit let-7 microRNA processing. It is highly expressed in human embryonic stem cells and has been used to enhance the generation of induced pluripotent stem cells.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
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Product information

Size
Order no.
Price

StemMACS Lin28 mRNA, human

  • For research use only
20 µg
130-101-117
EUR 254,00 

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