StemMACS™ Trilineage Differentiation Kit, human

StemMACS™ Trilineage Differentiation Kit, human

The StemMACS™ Trilineage Differentiation Kit enables directed differentiation of pluripotent stem cells into ecto-, meso- and endoderm. Combined with immunofluorescent or flow cytometric analysis it provides a simple 7-day assay for functional characterization of ES and iPS cell lines.

Background information

Pluripotency is the ability to differentiate into the three embryonal germ layers, ectoderm, mesoderm, and endoderm, and is a defining characteristic of pluripotent stem cells (PSCs). Therefore, basic characterization of PSC lines includes typically a test for pluripotency in addition to surface marker expression and morphology. However, traditional pluripotency assays such as embryoid body and teratoma formation are both time-consuming and difficult to quantitate.
The StemMACS Trilineage Differentiation Kit provides a functional assay that can be completed in 7 days. In contrast to other pluripotency assays, the StemMACS Trilineage Differentiation Kit allows either analysis by immunocytochemistry or quantitative analysis by flow cytometry. The kit is enabling parallel assessment of multiple hPSC lines. The 12-well format is optimal for flow cytometric analysis. For immunocytochemistry, the 24-well format may be sufficient.

Applications

Assessment of differentiation potential of human pluripotent stem cells.
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Product information

Size
Order no.
Price

StemMACS Trilineage Differentiation Kit, human

  • For research use only
for 12
assays
130-115-660
EUR 544,00 

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