Mouse M-CSF

Mouse M-CSF

M-CSF stands for macrophage-colony stimulating factor. Mouse M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse M-CSF can be used for a variety of applications, including:
  • Generation of bone marrow-derived macrophages.
  • Differentiation of osteoclasts.
  • Proliferation and apoptosis assays.

Background information

Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of M-NFS-60 cells
  • premium grade: ≥ 2×
    10
    5
    U/mg
  • research grade: ≥ 1×
    10
    5
    U/mg
  • Selected references

    1. Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
    2. Tanne, A. et al. (2009)
      A murine DC-SIGN homologue contributes to early host defense against
      Mycobacterium tuberculosis
      .
      J. Exp. Med. 206(10): 2205-2220
    3. Tanne, A. et al. (2009)
      A murine DC-SIGN homologue contributes to early host defense against
      Mycobacterium tuberculosis
      .
      J. Exp. Med. 206: 2205-2220
    4. Herold, J. et al. (2014) Tetanus toxoid-pulsed monocyte vaccination for augmentation of collateral vessel growth. J Am Heart Assoc. 3(2): e000611
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
-
E. coli
25 µg
-
E. coli
10 µg
-
E. coli
25 µg
-
E. coli
100 µg
-
E. coli
1000 µg
-