Mouse IL-4

Mouse IL-4

IL-4 stands for interleukin 4. Mouse IL-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-4 can be used for a variety of applications, including:
  • In vitro differentiation of naive CD4+ T cells towards TH 2 cells.
  • In vitro studies of bone marrow precursor development to DCs, together with GM-CSF.
  • In vitro investigation of B cell-T cell interaction, together with CD40-Ligand.

Background information

IL-4 is produced mainly by activated T
H
2 cells and, to a lesser extent, by T
H
1 cells. It binds to the IL-4 receptor α (CD124), followed by the dimerization with other receptor chains to generate type 1 and type 2 receptors. IL-4 promotes the proliferation and differentiation of activated B cells and the expression of MHC class II antigens. Mice over expressing IL-4 have elevated levels of IgE and IgG1 and show a deficiency in T cell maturation. CD4
+
T cells from knockout mice lacking IL-4 are not able to produce T
H
2 cytokines after
in vitro
stimulation. The biological activity of IL-4 is species-specific, i.e. murine IL-4 is inactive on human cells and
vice versa.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of HT-2 cells (NIBSC 91/656)
  • premium grade: ≥ 8×
    10
    6
    U/mg
  • research grade: ≥ 5×
    10
    6
    U/mg
Product options: 6

Product information

Size
Order no.
Price

Mouse IL-4, research grade

  • E. coli
  • For research use only
10 µg
130-094-061
EUR 135,00 

Mouse IL-4, research grade

  • E. coli
  • For research use only
25 µg
130-097-757
EUR 210,00 

Mouse IL-4, premium grade

  • E. coli
  • For research use only
10 µg
130-097-761
EUR 165,00 

Mouse IL-4, premium grade

  • E. coli
  • For research use only
25 µg
130-097-760
EUR 265,00 

Mouse IL-4, premium grade

  • E. coli
  • For research use only
100 µg
130-097-759
EUR 525,00 

Mouse IL-4, premium grade

  • E. coli
  • For research use only
1000 µg
130-097-758
EUR 2.220,00