Human SHH (C24II)

Human SHH (C24II)

SHH stands for sonic hedgehog. Human SHH (C24II) is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human SHH (C24II) can be used for a variety of applications including:
  • Expansion or differentiation of various cell types, mainly human and mouse embryonic stem cells, but also adult stem cells and mesenchymal cells.

Background information

Sonic hedgehog (SHH) is involved in a wide variety of embryonic developmental events, including neurogenesis, limb development, hematopoiesis, and gut formation. Besides their role as morphogens, hedgehog family members have also been shown to act as mitogens, cell survival factors, and axon guidance factors and to influence tissue regeneration within the adult. SHH is synthesized as a precursor, which is autocatalytically cleaved into a 19 kDa N-terminal domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N is responsible for the signaling activity of hedgehog proteins, while SHH-C acts as a cholesterol transferase and covalently attaches a cholesterol molecule to SHH-N. The lipid-modified SHH-N displays enhanced activity. Human SHH (C24II) corresponds to the mature SHH-N domain and carries a Cys to Ile-Ile substitution resulting in a lipophilic moiety and therefore an increased activity.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Alkaline phosphatase production by mouse C3H10T1/2 fibroblasts
  • premium grade: ≥ 2×
    10
    3
    U/mg
  • research grade: ≥ 1×
    10
    3
    U/mg
Product options: 6

Product information

Size
Order no.
Price

Human SHH (C24II), research grade

  • E. coli
  • For research use only
10 µg
130-095-717
EUR 135,00 

Human SHH (C24II), research grade

  • E. coli
  • For research use only
25 µg
130-095-718
EUR 210,00 

Human SHH (C24II), premium grade

  • E. coli
  • For research use only
10 µg
130-095-721
EUR 165,00 

Human SHH (C24II), premium grade

  • E. coli
  • For research use only
25 µg
130-095-723
EUR 265,00 

Human SHH (C24II), premium grade

  • E. coli
  • For research use only
100 µg
130-095-727
EUR 525,00 

Human SHH (C24II), premium grade

  • E. coli
  • For research use only
1000 µg
130-095-730
EUR 2.220,00 

Related products

5 products available | view all