Human IL-4

Human IL-4

IL-4 stands for Interleukin 4. Human IL-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-4 can be used for a variety of applications including:
  • In vitro generation of Mo-DCs together with GM-CSF.
  • In vitro differentiation of naive CD4+ T cells towards TH 2 cells.

Background information

Interleukin 4 (IL-4) is a pleiotropic cytokine and plays a central role in humoral and adaptive immune responses. IL-4 is predominantly secreted by activated CD4
+
memory and effector T
H
2 cells, basophils, and mast cells. It promotes the proliferation and differentiation of B cells, as well as immunoglobulin isotype switching, and MHC class II antigen and low-affinity IgE receptor expression. Furthermore, IL-4 induces the differentiation of naive CD4
+
T cells into helper T
H
2 cells, while suppressing T
H
1 development, and promotes chemotaxis of mast cells and basophils. Excessive IL-4 production and mechanisms involving T
H
2 types have been associated with immunological disorders, such as IgE-mediated allergy.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of TF-1 cells (NIBSC 88/656)
  • premium grade: ≥ 5×
    10
    6
    IU/mg
    (typical activity: ≥ 8×
    10
    6
    IU/mg
    )
  • research grade: ≥ 2×
    10
    6
    IU/mg
Product options: 9

Product information

Size
Order no.
Price

Human IL-4, research grade

  • E. coli
  • For research use only
5 µg
130-093-915
EUR 65,00 

Human IL-4, research grade

  • E. coli
  • For research use only
10 µg
130-095-373
EUR 100,00 

Human IL-4, research grade

  • E. coli
  • For research use only
25 µg
130-093-917
EUR 210,00 

Human IL-4, research grade

  • E. coli
  • For research use only
100 µg
130-094-117
EUR 420,00 

Human IL-4, premium grade

  • E. coli
  • For research use only
5 µg
130-093-919
EUR 85,00 

Human IL-4, premium grade

  • E. coli
  • For research use only
10 µg
130-093-920
EUR 130,00 

Human IL-4, premium grade

  • E. coli
  • For research use only
25 µg
130-093-921
EUR 265,00 

Human IL-4, premium grade

  • E. coli
  • For research use only
100 µg
130-093-922
EUR 525,00 

Human IL-4, premium grade

  • E. coli
  • For research use only
1000 µg
130-093-924
EUR 2.435,00 

Related products

9 products available | view all