Human IL-1β

Human IL-1β

IL-1β stands for interleukin-1 beta. Human IL-1β is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-1β can be used for a variety of applications including:
  • Induction of Mo-DC maturation.
  • Chemotaxis assays.
  • Investigation of IL-1 receptor signaling.

Alternative Names

IL1F2

Background information

Interleukin 1β (IL-1β) is a proinflammatory cytokine that is secreted mainly by monocytes and macrophages. IL-1β secretion has also been reported for a variety of other cells, including B cells, NK cells, dendritic cells, astrocytes, and microglial cells. It mediates inflammatory responses in B cells, T cells, and NK cells by inducing the production of cytokines, such as IL-2, IL-3, IL-6, as well as interferons. Upon exposure to IL-1β, endothelial cells and smooth muscle cells synthesize prostaglandins and other derivatives of arachidonic acid. In addition, IL-1β is found in synovial fluid of arthritis patients, causing degranulation of basophils and eosinophils as well as activation of osteoclasts. IL-1β is mitogenic for mesangial cells, glial cells, and keratinocytes. It has been shown that IL-1β is a potent modulator of CD40L-induced cytokine secretion by human dendritic cell (DC) subsets, such as monocyte-derived dendritic cells (MoDCs), CD34
+
-derived DCs, and peripheral blood DCs.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of D10.G4.1 cells (NIBSC 86/680)
  • premium grade: ≥ 3×
    10
    7
    IU/mg
  • research grade: ≥ 1×
    10
    7
    IU/mg
  • Selected References

    1. Cunin P. et al. (2011) The tachykinins substance P and hemokinin-1 favor the generation of human memory Tʜ17 cells by inducing IL-1β, IL-23, and TNF-like 1A expression by monocytes. J. Immunol. 186(7): 4175-4182
    2. Poole, S. and Gaines-Das, R. E. (1991) The international standards for interleukin-1 alpha and interleukin-1 beta. Evaluation in an international collaborative study. J. Immunol. Methods 142: 1-13
    3. Dussiau C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
    4. Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928
    5. Schipper H. S. et al. (2010) A multiplex immunoassay for human adipokine profiling. Clin. Chem. 56(8): 1320-1328
    6. Kitamura, T. et al. (1991) IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoetic cell line, TF-1. Int. Immunol. 3: 571-577
    7. Luft, T. et al. (2002) IL-1β enhances CD40 ligand-mediated cytokine secretion by human dendritic cells (DC): a mechanism for T cell-independent DC activation. J. Immunol. 168: 713-722
Product options: 6

Product information

Size
Order no.
Price

Human IL-1β, research grade

  • E. coli
  • For research use only
10 µg
130-093-895
EUR 155,00 

Human IL-1β, research grade

  • E. coli
  • For research use only
25 µg
130-095-374
EUR 290,00 

Human IL-1β, premium grade

  • E. coli
  • For research use only
10 µg
130-093-897
EUR 190,00 

Human IL-1β, premium grade

  • E. coli
  • For research use only
25 µg
130-093-563
EUR 365,00 

Human IL-1β, premium grade

  • E. coli
  • For research use only
100 µg
130-093-898
EUR 630,00 

Human IL-1β, premium grade

  • E. coli
  • For research use only
1000 µg
130-093-899
EUR 2.750,00