Human IL-12

Human IL-12

IL-12 stands for interleukin 12. Human IL-12 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-12 can be used for a variety of applications, including:
  • In vitro differentiation of naive CD4+ T cells towards TH 1 cells.
  • In vitro proliferation and stimulation of cytotoxic activity of NK cells and T cells.

Background information

Interleukin 12 (IL-12) is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation, and enhances the cytotoxic activity within these cell populations. Another important activity of IL-12, acting together with IFN-γ and IL-2, is to drive T helper cell responses toward the T
H
1 rather than the T
H
2 phenotype. Moreover, IL-12 is also important in resistance to viral disease and has significant antitumor activity. It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive
in vitro
and
in vivo
.
2,3

Quality description

Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Induction of IFN-γ secretion by human T cells (NIBSC 95/544)
  • premium grade: ≥ 3×
    10
    6
    U/mg
  • Selected References

    1. Wulff, H. et al. (2007) Cloning and characterization of an adenoviral vector for highly efficient and doxycycline-suppressible expression of bioactive human single-chain interleukin 12 in colon cancer. BMC Biotechnol. 7: 35
    2. Lieschke, G.J. et al. (1997)
      Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity
      in vivo.
      Nat. Biotechnol. 15: 35-40
    3. Miller, J.M. et al. (2010)
      Vesicular stomatitis virus modified with single chain IL-23 exhibits oncolytic activity against tumor cells
      in vitro
      and
      in vivo.
      Int. J. Infereron Cytokine Mediator Res. 2010: 63-72
    4. Juelke, K. et al. (2010)
      CD62L expression identifies a unique subset of polyfunctional CD56
      dim
      NK cells.
      Blood 116(8): 1299-1307
Product options: 3

Product information

Size
Order no.
Price

Human IL-12, premium grade

  • HEK293 cells
  • For research use only
5 µg
130-096-704
EUR 110,00 

Human IL-12, premium grade

  • HEK293 cells
  • For research use only
25 µg
130-096-705
EUR 335,00 

Human IL-12, premium grade

  • HEK293 cells
  • For research use only
100 µg
130-096-798
EUR 1.090,00