Human G-CSF

Human G-CSF

G-CSF stands for granulocyte colony stimulating factor. Human G-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human G-CSF can be used for a variety of applications, including:
  • Growth promotion and differentiation of cells of the neutrophil lineage.
  • Induction of colony formation of normal and leukemic bone marrow cells in soft agar cultures.

Background information

Granulocyte colony stimulating factor (G-CSF) is a hematopoietic growth factor that affects proliferation and differentiation especially of progenitors of the neutrophil and granulocyte lineages. It is produced mainly by monocytes and macrophages and a variety of other cells like astrocytes, fibroblasts and endothelial cells in response to specific stimulation, for instance by endotoxin, TNF-α and IFN-γ. Furthermore, G-CSF enhances the survival and influences the immunological functions of mature neutrophils. Thus, in addition to its properties as a hematopoietic growth factor G-CSF also acts as a mediator of host defense against infection and inflammatory response.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of NFS-60 cells (NIBSC 88/502)
  • premium grade: ≥ 4×
    10
    7
    IU/mg
  • research grade: ≥ 2×
    10
    7
    IU/mg
  • Selected references

    1. Shirafuji, N. et al. (1989) A new bioassay for human granulocyte colony-stimulating factor (hG-CSF) using murine myeloblastic NFS-60 cells as targets and estimation of its levels in sera from nomral healthy persons and patients with infectious and hematological disorders. Exp. Hematol. 17: 116-119
    2. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
Product options: 6
130-096-345

Human G-CSF, research grade

E. coli
10 µg
EUR 155,00 
130-096-346

Human G-CSF, research grade

E. coli
25 µg
EUR 290,00 
130-093-860

Human G-CSF, premium grade

E. coli
10 µg
EUR 190,00 
130-096-347

Human G-CSF, premium grade

E. coli
25 µg
EUR 365,00 
130-093-861

Human G-CSF, premium grade

E. coli
100 µg
EUR 840,00 
130-094-265

Human G-CSF, premium grade

E. coli
1000 µg
EUR 3.280,00