Human FGF-8b

Human FGF-8b

FGF-8b stands for fibroblast growth factor 8b. Human FGF-8b is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human FGF-8b can be used for a variety of applications, including:
  • Differentiation of embryonic stem cells into neutral progenitor cells.
  • Differentiation of embryonic stem cell-derived neural progenitor cells into dopaminergic neurons.
  • Survival of neural precursor cells and differentiation into astroglial cells.
  • Stimulation of osteoblast proliferation.
  • Proliferation of endothelial cells.
  • Proliferation of myogenic cells.
  • Tumor growth studies of prostate and breast cancer.
  • Protection of hippocampal cultures from oxidative stress.

Background information

Fibroblast growth factor 8 (FGF-8), originally identified as androgen-induced growth factor, is a heparin-binding protein, which has mitogenic and transforming activity. Eight secreted FGF-8 isoforms (FGF-8a to h) have been identified in mice, whereas four have been described in humans (a, b, e, and f). FGF-8b appears to be the predominant form and has been shown to have the most important oncogenic transforming capacity. FGF-8 exerts a pivotal role in embryogenesis. It is expressed during gastrulation and influences brain, limb, heart and facial development in the mouse embryo. In addition, FGF-8 was shown to stimulate osteoblast proliferation. The amino acid sequence of human FGF8-b shares 100% identity with mouse FGF-8b.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 3T3 cells
  • premium grade: ≥ 2×
    10
    4
    U/mg
  • research grade: ≥ 1×
    10
    4
    U/mg
Product options: 6

Product information

Size
Order no.
Price

Human FGF-8b, research grade

  • E. coli
  • For research use only
10 µg
130-095-731
EUR 100,00 

Human FGF-8b, research grade

  • E. coli
  • For research use only
25 µg
130-095-733
EUR 155,00 

Human FGF-8b, premium grade

  • E. coli
  • For research use only
10 µg
130-095-737
EUR 130,00 

Human FGF-8b, premium grade

  • E. coli
  • For research use only
25 µg
130-095-738
EUR 190,00 

Human FGF-8b, premium grade

  • E. coli
  • For research use only
100 µg
130-095-740
EUR 525,00 

Human FGF-8b, premium grade

  • E. coli
  • For research use only
1000 µg
130-095-741
EUR 2.220,00