Human FGF-2 IS

Human FGF-2 IS

FGF-2 IS stands for fibroblast growth factor 2 "Improved Sequence", also termed fibroblast growth factor basic (FGF-b) or basic FGF. Human FGF-2 IS is a variant of Human FGF-2 with a proprietary amino acid substitution. FGF-2 IS is protected by patent EP2930181. Human FGF-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human FGF-2 can be used for a variety of applications, including:
  • Stimulation of proliferation and differentiation of several cell types, such as mesenchymal stromal cells, neural cells, and endothelial cells.
  • Long-term maintenance and propagation of undifferentiated embryonic and induced pluripotent stem cells.
  • Differentiation of neural cells starting from embryonic and induced pluripotent stem cell cultures.

Alternative names

basic FGF

Background information

Human FGF-2 belongs to the family of heparin-binding growth factors. It functions as a wide-spectrum mitogenic, angiogenic, and neurotrophic factor and stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells. FGF-2 has been implicated in a multitude of physiological and pathological processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF-2 IS is an engineered FGF-2 variant with increased thermostability and higher resistance to proteases, and retains the same biological properties as naturally occurring FGF-2.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 3T3 cells (NIBSC 90/712)
  • premium grade: ≥ 2×
    10
    6
    IU/mg
  • research grade: ≥ 1×
    10
    6
    IU/mg
  • Selected references

    1. Robinson, C. J. and Gaines-Das, R. (1994)
      The international standard for basic fibroblast growth factor (FGF-2); comparison of candidate preparations by
      in vitro
      bioassays and immunoassays.
      Growth Factors 11: 9-16
Product options: 6
E. coli
10 µg
EUR 70,00 
E. coli
50 µg
EUR 190,00 
E. coli
10 µg
EUR 100,00 
E. coli
50 µg
EUR 265,00 
E. coli
200 µg
EUR 525,00 
E. coli
1000 µg
EUR 1.270,00