Specifications for Ly-49C/I Antibody, anti-mouse, REAfinity™
Overview
Clone REA253 recognizes the mouse lymphocyte antigen 49c (Ly-49C), a type II transmembrane protein which is also known as killer cell lectin-like receptor 3 (Klra3). The REA253 antibody reacts with Ly-49C of BALB, B6, NZB, C57BL, and Ly-49I of B6 mice strains. Ly-49 family members are both activating and inhibitory receptors and are mainly receptors for MHC class I molecules. Ly-49C is a cell surface molecule expressed on a subpopulation of murine natural killer cells and NK1.1 T cells that are involved in the specific rejection of H-2
d or H-2
f (hemopoietic histocompatibility determinant 2) bone marrow cell grafts. It transduces a negative signal to NK cells upon recognition of class I antigens. The particular allele of Ly-49C, i.e., C57BL versus BALB, determines to a large extent which class I antigen (H-2K
b for B6, K
b and H-2
d for BALB/c or BALB.B) sends negative signals to Ly-49C
+ NK cells. Clone REA253 blocks the binding of Ly-49C to MHC class I antigens of the b, d, k, and s haplotypes. The epitope recognized by REA253 may be masked on NK cells due to interactions with MHC class I molecules.
Additional information: Clone REA253 displays negligible binding to Fc receptors.
Alternative names
Klra3, Klra9Detailed product information
Technical specifications
| Clone | REA253 |
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| Clonality | monoclonal |
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| Isotype | recombinant human IgG1 |
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| Isotype control | REA Control Antibody, human IgG1 |
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| Host | human cell line |
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| Type of antibody | Primary antibodies, Recombinant antibodies |
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| Species | mouse |
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| Antigen | Ly-49C/I |
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| Alternative names of antigen | Klra3, Klra9 |
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| Molecular mass of antigen [kDa] | 31 |
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| Distribution of antigen | NK cells |
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| Entrez Gene ID | 16634, 16640 |
|---|
| RRID | AB_2652707, AB_2652708, AB_2652704, AB_2652705, AB_2652706, AB_2652703 |
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