Clone:
REAL1073
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
Lamin-B1, LMNB1, LMN2, LMNB

Specifications for Lamin B1 Antibody, anti-human, REAdye_lease™

Overview

Clone REAL1073 is an antibody fragment derived from the full Lamin B1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL1073 recognizes the protein Lamin B1, which belong to the lamin group and is a member of the the intermediate filament family. In vertebrates, two types of lamins exist, A and B. These evolutionarily highly conserved proteins are part of the nuclear lamina that rests on the inner nuclear membrane. During mitosis, lamin B1 is phosphorylated by the CDK1/cyclin B complex and then depolymerized, which leads to a decomposition of the nuclear lamina. Lamin B 1 regulates nuclear stability, chromatin structure and gene expression and plays an important role in cellular senescence. Lamins are also involved in a wide range of pathologies, including aging-associated diseases such as cardiovascular morbidities and cancer. For example it is reported, that the loss of lamin B1 plays an essential role in lung cancer or leads to systemic inflammation and gut hyperplasia.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

Lamin-B1, LMNB1, LMN2, LMNB

Detailed product information

Technical specifications

CloneREAL1073
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
Cross-reactivitymouse, rat
AntigenLamin B1
Alternative names of antigenLamin-B1, LMNB1, LMN2, LMNB
Distribution of antigenastrocytes, microglia, neurons, oligodendrocytes

References for Lamin B1 Antibody, anti-human, REAdye_lease™

Publications

  1. Chen, H. et al. (2014) Age-associated loss of lamin-B leads to systemic inflammation and gut hyperplasia. Aging (Albany NY) 129(4): 829-843
  2. de Leeuw, R. et al. (2018) Nuclear Lamins: Thin Filaments with Major Functions. Trends Cell Biol. 28(1): 34-45
  3. Jia, Y. et al. (2019) Lamin B1 loss promotes lung cancer development and metastasis by epigenetic derepression of RET. J. Exp. Med. 216(6): 1377-1395
  4. Boyan, K. et al. (2019) Lamin B1 in cancer and aging. Aging (Albany NY) 11(18): 7336-7338