Clone:
REA261
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
Killer cell lectin-like receptor subfamily G member 1, CLEC15A, MAFA, MAFA-2F1, MAFA-L, 2F1

Extended validation for KLRG1 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA261
13A2++
13F12F2++
SA231A2++
14C2A07+
Cells were incubated with an excess of purified unconjugated KLRG1 (REA261) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for KLGR1. Human peripheral blood mononuclear cells (PBMCs) were stained with KLGR1 antibodies and with a suitable counterstaining. As a control, KLGR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for KLGR1. Human peripheral blood mononuclear cells (PBMCs) were stained with KLGR1 antibodies and with a suitable counterstaining. As a control, KLGR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for KLGR1. Human peripheral blood mononuclear cells (PBMCs) were stained with KLGR1 antibodies and with a suitable counterstaining. As a control, KLGR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for KLGR1. Human peripheral blood mononuclear cells (PBMCs) were stained with KLGR1 antibodies and with a suitable counterstaining. As a control, KLGR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for KLGR1. Human peripheral blood mononuclear cells (PBMCs) were stained with KLGR1 antibodies and with a suitable counterstaining. As a control, KLGR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for KLGR1. Human peripheral blood mononuclear cells (PBMCs) were stained with KLGR1 antibodies and with a suitable counterstaining. As a control, KLGR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using KLRG1 (REA261). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using KLRG1 (REA261). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using KLRG1 (REA261). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for KLRG1 Antibody, anti-human, REAfinity™

Overview

Clone REA261 recognizes the killer cell lectin-like receptor subfamily G member 1 (KLRG1) antigen, a transmembrane protein, which is also known as C-type lectin domain family 15 member A (CLEC15A) or ITIM-containing receptor MAFA-L. KLRG1 is expressed by basophils, CD4 and CD8 T cells that exhibit a memory cell phenotype, and by a large proportion of peripheral blood NK cells. Expression of KLRG1 by virus-specific CD8
+
T cells is induced by repetitive antigen stimulation and it differs in chronic versus resolved viral infections. T cells expressing KLRG1 exhibit a poor proliferation potential, while their ability to secrete gamma interferon is preserved. Thus, KLRG1 is a convenient marker for early T cell–differentiation.
Additional information: Clone REA261 displays negligible binding to Fc receptors.

Alternative names

Killer cell lectin-like receptor subfamily G member 1, CLEC15A, MAFA, MAFA-2F1, MAFA-L, 2F1

Detailed product information

Technical specifications

CloneREA261
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenKLRG1
Alternative names of antigenKiller cell lectin-like receptor subfamily G member 1, CLEC15A, MAFA, MAFA-2F1, MAFA-L, 2F1
Molecular mass of antigen [kDa]22
Distribution of antigenT cells, NK cells, basophils
Entrez Gene ID10219
RRIDAB_2733432, AB_2752143, AB_2752091, AB_2784406, AB_2784405, AB_2784408, AB_2784407, AB_2784410, AB_2784409, AB_2784404, AB_2784403, AB_2752142, AB_2752090, AB_2811538, AB_2811530, AB_2733431

Resources for KLRG1 Antibody, anti-human, REAfinity™

Certificates

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References for KLRG1 Antibody, anti-human, REAfinity™

Publications

  1. Voehringer, D. et al. (2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1). Blood 100: 3698-3702
  2. Butcher, S. et al. (1998) MAFA-L, an ITIM-containing receptor encoded by the human NK cell gene complex and expressed by basophils and NK cells. Eur. J. Immunol. 28(11): 3755-3762
  3. Thimme, R. et al. (2005) Increased expression of the NK cell receptor KLRG1 by virus-specific CD8 T cells during persistent antigen stimulation. J. Virol. 79: 12112-12116

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