Clone:
REA201
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC
Alternative names:
IRF4, LSIRF, MUM1, NF-EM5, SHEP8, Spip, LSIRF13, ICSAT14

Extended validation for IRF-4 Antibody, anti-human/mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA201
IRF4.3E4++
3E4++
Q9-343-
Cells were incubated with an excess of purified unconjugated IRF-4 (REA201) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for IRF-4. Human peripheral blood mononuclear cells (PBMCs) were stimulated with 5µg/ml PHA for 3 days. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Cell signalling buffer set A followed by a staining with IRF-4 antibodies. As a control, IRF-4 antibody staining was omitted and cells were measured in the same channels.Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IRF-4. Human peripheral blood mononuclear cells (PBMCs) were stimulated with 5µg/ml PHA for 3 days. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Cell signalling buffer set A followed by a staining with IRF-4 antibodies. As a control, IRF-4 antibody staining was omitted and cells were measured in the same channels.Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IRF-4. Human peripheral blood mononuclear cells (PBMCs) were stimulated with 5µg/ml PHA for 3 days. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Cell signalling buffer set A followed by a staining with IRF-4 antibodies. As a control, IRF-4 antibody staining was omitted and cells were measured in the same channels.Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for IRF-4. Human peripheral blood mononuclear cells (PBMCs) were stimulated with 5µg/ml PHA for 3 days. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Cell signalling buffer set A followed by a staining with IRF-4 antibodies. As a control, IRF-4 antibody staining was omitted and cells were measured in the same channels.Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for IRF-4 Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA201 recognizes IRF-4, which is a 52 KDa transcription factor and belongs to the family of interferon regulatory transcription factors (IRF).The IRF family consists of nine members in both human and mice and is characterized by a conserved N-terminal DNA-binding domain (DBD) with unique tryptophan pentad repeat and modulates the expression of genes regulated by the presence of interferons and in response to cell activation. Expression of IRF-4 is found in cells of the immune system, including lymphocytes, dendritic cells, and macrophages, where it's multiple functions include regulation of cell proliferation, apoptosis, and differentiation. Transcriptional activity of IRF-4 is further modulated by its interaction with various co-factors including including Pu.1, E47, STAT-6, and IRF-8.
Additional information: Clone REA201 displays negligible binding to Fc receptors.

Alternative names

IRF4, LSIRF, MUM1, NF-EM5, SHEP8, Spip, LSIRF13, ICSAT14

Detailed product information

Technical specifications

CloneREA201
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenIRF-4
Alternative names of antigenIRF4, LSIRF, MUM1, NF-EM5, SHEP8, Spip, LSIRF13, ICSAT14
Molecular mass of antigen [kDa]52
Distribution of antigenT cells, B cells, macrophages, dendritic cells
Entrez Gene ID3662
RRIDAB_2652516, AB_2652517, AB_2652518, AB_2652519

Resources for IRF-4 Antibody, anti-human/mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for IRF-4 Antibody, anti-human/mouse, REAfinity™

Publications

  1. Klein, U. et al. (2006) Transcription factor IRF4 controls plasma cell differentiation and class-switch recombination. Nat. Immunol. 7(7): 773-782
  2. Biswas, P. S. et al. (2012) Dual regulation of IRF4 function in T and B cells is required for the coordination of T–B cell interactions and the prevention of autoimmunity. J. Exp. Med. 209: 581-596
  3. Nayar, R. et al. (2012)
    TCR signaling via Tec kinase ITK and interferon regulatory factor 4 (IRF4) regulates CD8
    +
    T-cell differentiation.
    Proc. Natl. Acad. Sci. U.S.A. 109: E2794- E2802

Related products for
IRF-4 Antibody, anti-human/mouse, REAfinity™

4 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?