IL-1RAcP Antibody, anti-human, REAfinity™

Name: IL-1RAcP Antibody, anti-human, REAfinity™
Availability: InStock

IL-1RAcP Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA558

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

FC

Alternative names:

C3orf13, IL1R3

Flow cytometry applications forIL-1RAcP Antibody, anti-human, REAfinity™

APC
APC-Vio 770
Biotin
PE
PE-Vio 770
Vio Bright FITC
Vio Bright V423

Conjugate:

APC

Recommended dilution:

1:11

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-108-755, 130-108-688

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:11)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IL-1RAcP antibodies or with the corresponding REA Control (S) antibodies (left image) as well as with CD14 antibodies. Flow cytometry was performed using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Extended validation for IL-1RAcP Antibody, anti-human, REAfinity™

Specificity

Knockout validation

To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.

WT

KO

Fluorescence microscopy image of IL-1RAcP knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with IL-1RAcP-PE (REA558, red) and counterstained with DRAQ5 (blue) as DNA stain.

Fluorescence microscopy image of IL-1RAcP knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with IL-1RAcP-PE (REA558, red) and counterstained with DRAQ5 (blue) as DNA stain.

Overlay histogram showing flow cytometric analysis of IL-1RAcP knockout cells. Wild type (red) and knockout cells (blue) were stained with IL-1RAcP-PE, clone (REA558). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Overlay histogram showing flow cytometric analysis of IL-1RAcP knockout cells. Wild type (red) and knockout cells (blue) were stained with IL-1RAcP-PE, clone (REA558). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for IL-1RAcP Antibody, anti-human, REAfinity™

Overview

Clone REA558 recognizes the human IL-1RAcP antigen. The IL-1 receptor accessory protein (IL-1RAcP) is the second subunit required to form a functional receptor complex for IL-1α and β, IL-1F6, IL-1F8, IL1-F9, and IL-33 and is necessary to mediate signal transduction. IL-1RAcP is found in a variety of tissues.

Additional information: Clone REA558 displays negligible binding to Fc receptors

Alternative names

C3orf13, IL1R3

Detailed product information

Technical specifications

Clone	REA558
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (S), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	IL-1RAcP
Alternative names of antigen	C3orf13, IL1R3
Molecular mass of antigen [kDa]	63
Entrez Gene ID	3556
RRID	AB_2652387, AB_2652388, AB_2652389, AB_2652390, AB_2652391, AB_2652392, AB_2652393, AB_2652394, AB_2652395, AB_2922156, AB_2922122, AB_2928713, AB_2928712, AB_2652386

Resources for IL-1RAcP Antibody, anti-human, REAfinity™

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for IL-1RAcP Antibody, anti-human, REAfinity™

Publications

Mansur, I. G. et al. (2011) Engagement of IL-1 receptor accessory protein (IL-1RAcP) with the monoclonal antibody AY19 provides co-activating signals and prolongs the CD2-induced proliferation of peripheral blood lymphocytes. Immunol. Lett. 139(1-2): 52-57
Stansberg, C. et al. (2005)
Cloning of the Atlantic salmon (
Salmo salar
) IL-1 receptor associated protein.
Fish Shellfish Immunol. 19(1): 53-65

Applications

Extended validation