Recombinant human M-CSF can regulate the proliferation, differentiation, and survival of monocytes, macrophages, osteoclasts and their hematopoietic progenitors . The macrophage colony-stimulating factor (M-CSF) is a potent hematopoietic cytokine that is involved in diverse processes, such as regulation of inflammatory responses, bone resorption, atherosclerosis or brain and placental development. M-CSF recombinant protein can be especially used in differentiation studies, cell culture and functional assays.

Data and images for Human M-CSF

Figures

Figure 1

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Human M-CSF activity assay.
The biological activity of Human M-CSF is determined by proliferation assay using NFS-60 cells.

Figure 1

Human M-CSF activity assay.
The biological activity of Human M-CSF is determined by proliferation assay using NFS-60 cells.

Figure 2

View details
SDS-PAGE of Human M-CSF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human M-CSF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Specifications for Human M-CSF

Overview

Recombinant human M-CSF can regulate the proliferation, differentiation, and survival of monocytes, macrophages, osteoclasts and their hematopoietic progenitors . The macrophage colony-stimulating factor (M-CSF) is a potent hematopoietic cytokine that is involved in diverse processes, such as regulation of inflammatory responses, bone resorption, atherosclerosis or brain and placental development. M-CSF recombinant protein can be especially used in differentiation studies, cell culture and functional assays.

Applications

Human M-CSF can be used for a variety of applications, including:
  • Survival studies and apoptosis assays, for example, using peripheral blood monocytes.
  • Differentiation of macrophages from peripheral blood monocytes.
  • Differentiation of osteoclasts from CD14+ monocytes.

Detailed product information

Background information

Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

Biological activity

  • Proliferation of NFS-60 cells (NIBSC 89/512)
  • research grade: ≥ 1×
    10
    7
    IU/mg
  • premium grade: ≥ 2×
    10
    7
    IU/mg
    (Typical specific activity: ≥ 1.2×
    10
    8
    IU/mg
    )
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human M-CSF

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Reviews for Human M-CSF

Macrophage Differentiation Using M-CSF

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Human M-CSF, premium grade (130-096-485)

Our aim was to differentiate macrophages from human PBMCs using M-CSF in different concentrations.

Macrophage Differentiation Using M-CSF

  • 1
  • 2
  • 3
  • 4
  • 5

Human M-CSF, premium grade (130-096-489)

Our aim was to differentiate macrophages from human PBMCs using M-CSF in different concentrations.

Macrophage Differentiation Using M-CSF

  • 1
  • 2
  • 3
  • 4
  • 5

Human M-CSF, premium grade (130-096-492)

Our aim was to differentiate macrophages from human PBMCs using M-CSF in different concentrations.

Macrophage Differentiation Using M-CSF

  • 1
  • 2
  • 3
  • 4
  • 5

Human M-CSF, premium grade (130-096-493)

Our aim was to differentiate macrophages from human PBMCs using M-CSF in different concentrations.

References for Human M-CSF

Publications

  1. Hussen, K. A. et al. (2017) Molecular and Functional Characterization of Lymphoid Progenitor Subsets Reveals a Bipartite Architecture of Human Lymphopoiesis. Immunity 47(4): 680-696
  2. Lahaye, X. et al. (2018) NONO Detects the Nuclear HIV Capsid to Promote cGAS-Mediated Innate Immune Activation. Cell 175(2): 488-501
  3. Barosova, H. et al. (2020) Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment. J. Vis. Exp. (159)
  4. Queval, C. J. et al. (2014) A microscopic phenotypic assay for the quantification of intracellular mycobacteria adapted for high-throughput/high-content screening. J. Vis. Exp. (83)
  5. Wu, D. J. et al. (2014) A novel in vivo gene transfer technique and in vitro cell based assays for the study of bone loss in musculoskeletal disorders. J. Vis. Exp. (88): 51810
  6. Hazlett, H. F. et al. (2020) Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival. Sci Rep 10(1): 10935
  7. Giroud, P. et al. (2020) Expression of TAM-R in Human Immune Cells and Unique Regulatory Function of MerTK in IL-10 Production by Tolerogenic DC. Front Immunol 11: 564133
  8. Dahlem, C. et al. (2020) Thioholgamide A, a New Anti-Proliferative Anti-Tumor Agent, Modulates Macrophage Polarization and Metabolism. Cancers (Basel) 12(5): 1288
  9. Fernandes, M. C. et al. (2016) Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures. mBio. 7(3)
  10. Kusao, I. et al. (2021) Cognitive performance related to HIV-1-infected monocytes. J Neuropathol Exp Neurol. 24(1): 71-80
  11. Mire-Sluis, A. R. et al. (1995) The international standard for macrophage colony stimulating factor (M-CSF). Evaluation in an international collaborative study. J. Immunol. Methods 179: 141-151
  12. Michelet, X. et al. (2015) MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b. J. Immunol. 194(5): 2079-2088
  13. Vogel, S. Z. et al. (2015)
    TCAIM decreases T cell priming capacity of dendritic cells by inhibiting TLR-induced Ca
    2+
    influx and IL-2 production.
    J. Immunol. 194(7): 3136-3146
  14. Guery, L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
  15. McEwan, W. A. et al. (2013) Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21. Nat. Immunol. 14(4): 327-336
  16. Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response. Proc. Natl. Acad. Sci. U.S.A. 109(49): 20071-20076
  17. Wang, C. et al. (2010)
    Innate immune response to
    Mycobacterium tuberculosis
    Beijing and other genotypes.
    PLoS One 5(10): e13594
  18. Meissner, F. et al. (2010) Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease. Blood 116(9): 1570-1573

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